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AgPath-ID™ One-Step RT-PCR Reagents

描述

AgPath-ID™ 一步法RT-PCR试剂盒利用一种快速单管实时荧光定量逆转录PCR方法ฃฌ可灵敏稳定地扩增RNA靶点กฃ

• 推荐用于RNA病原体扩增
• 一致的RNA靶位扩增ฃฌ高特异性ฃฌ高灵敏度
• 已经过优化ฃฌ与靶位特异性引物和探针一同使用
• 含有ROXฃฌ可用于定量荧光信号的标准化

AgPath-ID™ AgPath-ID™ 一步法 RT-PCR试剂配置用于快速简单地设置反应กฃ所有反应集于一管ฃฌ最大程度降低样本处理错误ฃฌ缩短设置时间กฃ组装好之后ฃฌ约1小时内即可获得结果กฃ试剂盒中的RT-PCR酶混合液组分有高效的 ArrayScript™ 逆转录酶กข可提供高产量cDNA的突变MMLV逆转录酶和用于特异性靶点扩增的热启动DNA聚合酶—AmpliTaq Gold® 聚合酶กฃ2X RT-PCR缓冲液包含有优化的试剂ฃฌ用于高效稳定地进行的逆转录和PCRฃป缓冲液中还含有专利参比荧光ROXฃฌ用于定量荧光信号的标准化กฃ该试剂盒还提供了可选的检测增强剂ฃฌ可扩增具有富GC区或顽固二级结构的模板กฃ
For Research Use Only. Not for use in diagnostic procedures.

规格

PCR Method: 1-Step qRT-PCR
Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
Polymerase: AmpliTaq Gold® DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
Platform: 7500 System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Comments: Works on all AB platforms, but is optimized for the 7500.
Product Size: 100 reactions
Shipping Condition: Dry Ice

内容及储存

Sufficient for 100 reactions of 25 µL, contains:
• 1375µL 2X RT-PCR Buffer
• 110µL 25X RT-PCR Enzyme Mix
• 190µL Detection Enhancer
• 1.75mL Nuclease-free Water.

上一篇ฃบMagMAX™-96 Viral RNA Isolation Kit 下一篇?#22909;?#26377;了


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