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AgPath-ID™ One-Step RT-PCR Reagents

描述

AgPath-ID™ 一步法RT-PCR試劑盒利用一種快速單管實時熒光定量逆轉錄PCR方法,可靈敏穩定地擴增RNA靶點。

• 推薦用于RNA病原體擴增
• 一致的RNA靶位擴增,高特異性,高靈敏度
• 已經過優化,與靶位特異性引物和探針一同使用
• 含有ROX,可用于定量熒光信號的標準化

AgPath-ID™ AgPath-ID™ 一步法 RT-PCR試劑配置用于快速簡單地設置反應。所有反應集于一管,最大程度降低樣本處理錯誤,縮短設置時間。組裝好之后,約1小時內即可獲得結果。試劑盒中的RT-PCR酶混合液組分有高效的 ArrayScript™ 逆轉錄酶、可提供高產量cDNA的突變MMLV逆轉錄酶和用于特異性靶點擴增的熱啟動DNA聚合酶—AmpliTaq Gold® 聚合酶。2X RT-PCR緩沖液包含有優化的試劑,用于高效穩定地進行的逆轉錄和PCR;緩沖液中還含有專利參比熒光ROX,用于定量熒光信號的標準化。該試劑盒還提供了可選的檢測增強劑,可擴增具有富GC區或頑固二級結構的模板。
For Research Use Only. Not for use in diagnostic procedures.

規格

PCR Method: 1-Step qRT-PCR
Reverse Transcriptase: ArrayScript™ Reverse Transcriptase
Polymerase: AmpliTaq Gold® DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
Platform: 7500 System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Comments: Works on all AB platforms, but is optimized for the 7500.
Product Size: 100 reactions
Shipping Condition: Dry Ice

內容及儲存

Sufficient for 100 reactions of 25 µL, contains:
• 1375µL 2X RT-PCR Buffer
• 110µL 25X RT-PCR Enzyme Mix
• 190µL Detection Enhancer
• 1.75mL Nuclease-free Water.

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