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朗報®LAMP® 沙門氏菌核酸檢測試劑盒

朗報®LAMP® 沙門氏菌核酸檢測試劑盒

(環介導等溫擴增法)

測定原理及方法

對食品等檢驗對象進行前增菌預培養后,通過堿性熱處理法提取DNA,作為試樣溶液,進行環介導等溫擴增法反應。當存在引物可識別的沙門氏菌屬保守的侵入性相關基因invA6的核酸序列時,試樣溶液中的DNABst DNA聚合酶的作用下特異性擴增。通過核酸擴增的副產物——焦磷酸鎂(白色沉淀物)的濁度變化,可對核酸擴增進行檢測,進而可判定是否存在沙門氏菌屬。

   

  

 

   




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