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朗报®LAMP® 沙门氏菌核酸检测试剂盒

朗报®LAMP® 沙门氏菌核酸检测试剂盒

ฃจ环介导等温扩增法ฃฉ

测定原理及方法

对食品等检验对象进行前增菌预培养后ฃฌ通过碱性热处理法提取DNAฃฌ作为试样溶液ฃฌ进行环介导等温扩增法反应กฃ当存在引物可识别的沙门氏菌属保守的侵入性相关基因invA6ฃฉ的核酸序列?#20445;?#35797;样溶液中的DNABst DNA聚合酶的作用下特异性扩增กฃ通过核酸扩增的副产物——焦磷酸镁ฃจ白色沉淀物ฃฉ的浊度变化ฃฌ可对核酸扩增进行检测ฃฌ进而可判定是否存在沙门氏菌属กฃ

   

  

 

   




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